Journal: Animals : an Open Access Journal from MDPI

Article Title: Changes in the Mitochondrial Dynamics and Functions Together with the mRNA/miRNA Network in the Heart Tissue Contribute to Hypoxia Adaptation in Tibetan Sheep

doi: 10.3390/ani12050583

Figure Lengend Snippet: The primer information on mitochondria dynamic-related genes.

Article Snippet: The rabbit polyclonal anti OPA1 (bs-11764R, 1:100; Bioss, Beijing, China), Mic60 (bs-1824R, 1:100; Bioss, Beijing, China), MFF (bs-7628R, 1:100; Bioss, Beijing, China), Drp1 (bs-4100R, 1:100; Bioss, Beijing, China), and Fis1 (bs-7646R, 1:100; Bioss, Beijing, China) were added to the sections and incubated at 4 °C overnight, then incubated with goat anti-rabbit secondary antibody IgG/horseradish peroxide (HRP) (GB23303, 1:200; Servicebio, Wuhan, China) for 50 min at 25 °C.

Techniques: Sequencing

Journal: Animals : an Open Access Journal from MDPI

Article Title: Changes in the Mitochondrial Dynamics and Functions Together with the mRNA/miRNA Network in the Heart Tissue Contribute to Hypoxia Adaptation in Tibetan Sheep

doi: 10.3390/ani12050583

Figure Lengend Snippet: The expression of mitochondria fission ( Drp1 ( a ), Fis1 ( b ), MFF ( c )) and fusion ( Mic60 ( d ), OPA1 ( e )) genes in five tissues of Tibetan sheep. ( 1–5 ) Heart, liver, lung, brain, quadriceps femoris. ( TS25 ) 2500 m altitude Tibetan sheep. ( TS35 ) 3500 m altitude Tibetan sheep. ( TS45 ) 4500 m altitude Tibetan sheep. Date shown on graph are means ± SEM. * p < 0.05, ** p < 0.01.

Article Snippet: The rabbit polyclonal anti OPA1 (bs-11764R, 1:100; Bioss, Beijing, China), Mic60 (bs-1824R, 1:100; Bioss, Beijing, China), MFF (bs-7628R, 1:100; Bioss, Beijing, China), Drp1 (bs-4100R, 1:100; Bioss, Beijing, China), and Fis1 (bs-7646R, 1:100; Bioss, Beijing, China) were added to the sections and incubated at 4 °C overnight, then incubated with goat anti-rabbit secondary antibody IgG/horseradish peroxide (HRP) (GB23303, 1:200; Servicebio, Wuhan, China) for 50 min at 25 °C.

Techniques: Expressing

Journal: Animals : an Open Access Journal from MDPI

Article Title: Changes in the Mitochondrial Dynamics and Functions Together with the mRNA/miRNA Network in the Heart Tissue Contribute to Hypoxia Adaptation in Tibetan Sheep

doi: 10.3390/ani12050583

Figure Lengend Snippet: Immunostaining of mitochondria fission (Drp1 ( A ), Fis1 ( B ), MFF ( C )) and fusion (Mic60 ( D ), OPA1 ( E )) proteins in five tissues of Tibetan sheep (20×). ( a – c ) Heart. ( d – f ) Liver. ( g – i ) Lung. ( j – l ) Brain. ( m – o ) Quadriceps femoris. ( 1 – 5 ) Heart, liver, lung, brain, quadriceps femoris. ( TS25 ) 2500 m altitude Tibetan sheep. ( TS35 ) 3500 m altitude Tibetan sheep. ( TS45 ) 4500 m altitude Tibetan sheep. Date shown on graph are means ± SEM. The IOD in heart of TS25 as control, * p < 0.05, ** p < 0.01.

Article Snippet: The rabbit polyclonal anti OPA1 (bs-11764R, 1:100; Bioss, Beijing, China), Mic60 (bs-1824R, 1:100; Bioss, Beijing, China), MFF (bs-7628R, 1:100; Bioss, Beijing, China), Drp1 (bs-4100R, 1:100; Bioss, Beijing, China), and Fis1 (bs-7646R, 1:100; Bioss, Beijing, China) were added to the sections and incubated at 4 °C overnight, then incubated with goat anti-rabbit secondary antibody IgG/horseradish peroxide (HRP) (GB23303, 1:200; Servicebio, Wuhan, China) for 50 min at 25 °C.

Techniques: Immunostaining

Journal: Journal of Clinical Medicine

Article Title: Osteochondritis Dissecans (OCD)-Derived Chondrocytes Display Increased Senescence, Oxidative Stress, Chaperone-Mediated Autophagy and, in Co-Culture with Adipose-Derived Stem Cells (ASCs), Enhanced Expression of MMP-13

doi: 10.3390/jcm8030328

Figure Lengend Snippet: Assessment of mitochondrial dynamics and mitophagy. HE and OCD chondrocytes were analysed by RT-PCR for mRNA expression. Expression of MNF ( A ), MIEF1 ( B ) and MIEF2 ( C ) was established in order to compare intensity of mitochondrial fusion in investigated groups. To analyse mitochondrial fission, FIS expression was investigated ( D ). Moreover, expression of mitophagy markers: PINK ( E ) and PARKIN ( F ) was analysed. Mitochondrial fission factor (MFF) protein level was established by Western blot ( G ). Results expressed as mean ± S.D. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: Finally membranes were incubated overnight with primary antibody for mitochondrial fission factor MFF (Biorbyt orb325479) at a dilution of 1:500.

Techniques: Reverse Transcription Polymerase Chain Reaction, Expressing, Western Blot